Bacteria and Types of Prokaryotes - MCAT Biological and Biochemical Foundations of Living Systems

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Question

Gram staining prepares bacteria to be visualized under light microscope.

What allows a bacteria to stain positively with gram stain?

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Answer

Gram-positive bacteria are able to retain the crystal violet stain found in gram stain because of their thick peptidoglycan cell walls. Consequently, these bacteria stain dark blue or purple by gram staining. Alternatively, gram-negative bacteria appear red or pink because they are unable to retain the dye due to thinner cell walls and less peptidoglycan.

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